Effect of acrylonitrile on proliferation and differentiation of rat embryo spinal cord nerve cell

  Objective   To observe the effect on spinal nerve cell of rat embryo by plating acrylonitrile(ACN).

  Methods   ACN solution(0.01, 0.1, 1.0, 10.0, 50.0, 100.0, 200.0 μg/ml)were put into primary spinal nerve cell of rat embryo of dawing been cultured 16 days; the concentration rates were conrted; the content of protein of malondialdehyde(MDA)and activity of superoxide dismutase(SOD)were determined.

  Results   Acrylintrile inhibited differentiation and proliferation spinal nerve cell of rat embryo.The concentration rate and dimension of cell were reduced.The concenration of 50% inhibitions of diffferentiation(ICD₅₀)was 29μg/ml.The concentration of 50% inhibitions of cells proliferation(ICV₅₀)was 42 μg/mL.

  Conclusion   The inhibition effect of acrylonitrile on spinal nerve cell of rat embryo might be associated with impact of protein synthetis and with causing lipid peroxidation.