Analysis on hypervariable sites in mitochondrial DNA of malignant transformed cells induced by trans-BPDE and nickel sulfide

FU Juan; JIANG Yiguo; CHEN Jiakun

doi:10.11847/zgggws2006-22-07-29

FU Juan, JIANG Yiguo, CHEN Jiakun, . Analysis on hypervariable sites in mitochondrial DNA of malignant transformed cells induced by trans-BPDE and nickel sulfide[J]. Chinese Journal of Public Health, 2006, 22(7): 816-817. DOI: 10.11847/zgggws2006-22-07-29

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Analysis on hypervariable sites in mitochondrial DNA of malignant transformed cells induced by trans-BPDE and nickel sulfide

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Objective To explore the alteration of target molecular sequences in the noncoding mitochondrial DNA(mtDNA)contr ol region in 16HBE cells transformed by carcinog enstrans-BPDE and crystalline nickel sulfide.
Methods The sequences of mtDNAcontrol region in 16HBE cells were detected by PCR-SSCP assay and sequencing.
Results Compared with those of control 16HBE cells, no change in hypervariable segments of mtDNA in malignant transformed cells and tumorigenic cells induced by trans-BPDE and crystalline nickel sulfide was detected.
Conclusion The noncoding mtDNA control region might not be a target sequence in carcinog enesis induced by chemical carcinogen.

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Analysis on hypervariable sites in mitochondrial DNA of malignant transformed cells induced by trans-BPDE and nickel sulfide

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