Effects of Ang-2 and tee polyphenols on VEC secreting ET in hypoxia condition

  Objective   To explore the effects of Angiotensin-2(Ang-2)on Vascular endothelial cell(VEC)secreting endothelin(ET)in hypoxia condition and protection of tee polyphenols(TPs).

  Methods   VEC were divided into 6 groups: control group: simple hypoxia group; Ang-2 group; hypoxia+Ang-2 group; hypoxia+Ang-2+high concentration TPs group; hypoxia+Ang-2+low concentration TPs group.All groups except control group and simple Ang-2 group were underwenthypoxia which was produced by hypobaric chamber.The ET content before hypoxia and 0.5 h, 6 h as well as 24 h after hypoxia were measured with radioimmunoassay.

  Results   (1) Simple hypoxia and simple Ang-2 promoted the VEC secreting ET significantly(P < 0.01);(2)TPs had the effect to inhibit the VEC secreting ET induced by hypoxia combined Ang-2(P < 0.01);The ET content of low concentration TPs was significant lower than that of the high concentration TPs at time of 6 h and 24 h(P < 0.01).

  Conclusion   Ang-2 can significantly increase the ET release of VEC in hypoxia condition.TPs can decrease the VEC secreting ET induced by hypoxia combined Ang-2, and the protective effects of low concentration TPs is better.