Objective To investigate the effect of lycopene on growth of human gastric carcinoma cells in vitro .
Methods The human gastric carcinoma SGC-7901 cells were treated with lycopene.IC50 of lycopene on SGC-7901 cells calculated by 3-(4, 5-dimethylthiazole-2-yl)-2, 5 diphenyl tetrazoliumbromide(MTT)assay.The rate of growth suppression on SGC-7901 cells was evaluated by cell growth curve and clone formation.The effect of lycopene on DNA synthesis of human gastric carcinoma SGC-7901 cells was evaluated by 3H-thymidine(3H-TdR)incorporation.Cell morphology was observed by inverted microcope.
Results Lycopene had significantly inhibitory effect on growth of human gastric carcinoma SGC-7901 cells, IC50 of 24, 48, 72, 96, 120, 148, 172h were 1.0×10-4, 9.2×10-5, 2.6×10-5, 4.8×10-5, 315×10-5, 413×10-5 and 3.1×10-5 mol/L, respectively.Treated with 0.3×10-4, 015×10-4、10-4 mol/L lycopene for 172 h, the rate of cells growth suppression was 64.75%, 79.02%, 87.13%, respectively.Treated with 0.3×10-4, 0.5×10-4, 10-4 mol/L lycopene for 24 h the rate of cloning formation of SGC-7901 cells was(30.2±1.9)%, (22.0±2.1)%, (16.9±2.0)%, respectively.Treated with 0.3×10-4, 0.5×10-4, 10-4 mol/L lycopene for 48 h, 3H-TdR in corporation of human gastric carcinoma SGC-7901 cells were(3757.06±114.27), (3148.24±190.23), (2408.32±155.83)cpm, respectively, which showed significantly decreased(P < 0.01), as compared with that in negative control group which were(5470.25±217.16) cpm; light absorption(value A)of human gastric carcinoma SGC-7901 cells also showed significant decrease(P < 0.01), as compared with that in negative control group.The morphological changes of human gastric carcinoma SGC-7901 cells were observed after treated with 0.3×10-4, 0.5×10-4, 10-4 mol/L lycopene for 24 h, such as rounder, smaller, desquamation, sparse arrangement, vacuole of different size and etc.
Conclusion Lycopene can significantly inhibit proliferation of human gastric carcinoma SGC-7901 cells in dose-response manner in vitro .
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