Gene chip screen for gene expression pattern in 16HBE cells treated with BPDE

  Objective   To screen the differentially expressed genes in human bronchial epithelial cell line(16 HBE)after treatment with dihydroxyepoxy benzo pyrene(BPDE)by cDNAmicroarray.

  Methods   16 HBEcells and 16 HBEcells were treated with BPDEonly.The total RNAs were extracted from these cells.The cDNAprobes were prepared by labeling with Cy3-dCTPand Cy5-dCTPrespectively through reverse transcription.The mixed probes were then hybridized to the cDNAmicroarray chips containing 4096 human genes.The chips were scanned by ScanArray 4000 laser scanner.The acquired fluorescent signals were analyzed by GenPix Pro 3.0 software.Bioinformatical analysis of those differentially expressed genes had been performed.

  Results   143 genes exhibited differential expression between cells treated with BPDEonly and 16 HBE.The expression of 91 genes(63.63%)was down-regulated and of 52 genes(36.36%)was up-regulated.

  Conclusion   The regulation of genes including stress response genes, immune related genes, DNAsynthesis and repair genes Metabolism genes may be involved in itransforming activity of BPDE.