Objective To study the effects of dipfluzine(Dip)against lead-induced injury in primary cultured hippocampal neurons and explore its mechanism.
Methods The primary cultured hippocampal neurons were treated with PbAc2 to establish injury model.The cell viability, lactate dehy drogenase(LDH)leakage rate and intr acellularCa2+i in cultures were measured to study the protective effect of Dip.
Results Dip(1.0 μmol/Land 10 μmol/L)could lighten the damage of cultured hippocampal neurons induced by lead.Dip(1.0Lmol/Land 10 μmol/L)incr eased the cell viability and decreased the LDHleakage rate of cultured hippocampal neurons(P < 0.01).Dip(0.1 μmol/L, 1.0 μmol/Land 10 μmol/L)could decrease intracellularCa2+i (P < 0.01 or P < 0.05).
Conclusion Dip holds protective effect against lead-induced injury in cultured hippocampal neurons, which is possibly due to inhibiting the intr acellular calcium overload in neurons.