Protective effect of dipfluzine on lead-induced injury in cultured primary hippocampal neurons

  Objective   To study the effects of dipfluzine(Dip)against lead-induced injury in primary cultured hippocampal neurons and explore its mechanism.

  Methods   The primary cultured hippocampal neurons were treated with PbAc₂ to establish injury model.The cell viability, lactate dehy drogenase(LDH)leakage rate and intr acellularCa²⁺_i in cultures were measured to study the protective effect of Dip.

  Results   Dip(1.0 μmol/Land 10 μmol/L)could lighten the damage of cultured hippocampal neurons induced by lead.Dip(1.0Lmol/Land 10 μmol/L)incr eased the cell viability and decreased the LDHleakage rate of cultured hippocampal neurons(P < 0.01).Dip(0.1 μmol/L, 1.0 μmol/Land 10 μmol/L)could decrease intracellularCa²⁺_i (P < 0.01 or P < 0.05).

  Conclusion   Dip holds protective effect against lead-induced injury in cultured hippocampal neurons, which is possibly due to inhibiting the intr acellular calcium overload in neurons.