Analysis on gene typing of Salmonella paratyphi A in Ningbo city

  Objective   To investigate the dominant gene type of Salmonella paratyphi Ain Ningbo bypulsed-field gelelectrophoresis(PFGE), and to provide a way to distinguish the causing pathogens in the epido miologic investigations.

  Methods   The biolog ical character istics and sero-types were determined by Vitek32 and serolog ical test, respectively.Chromosomal gene patterns were typed by PFGE.

  Results   102 strains salmonella p ar atyp hi Awere divided into 9 PFGEtypes: 73 strians in X2 type(accounting for 71.57%), 21 in X1 type(20.58%), 2 in X7 type(1.96%), while 1 in the each other type(0.9%, respectively).The dominant type was X2.The PFGEDNAfingerpr ints differed among 1 to 3 bands, which demonstr ated that little mutat ion existed in Salmonella paratyphi Astrains in Ningbo, suggesting that all stratus maybe belong to the same clone of Sal monella paratyphiA.

  Conclusion   PFGEtyping plays a vital role in surveillance for and the investigation of the outbreaks of Sal monella par atyp hi Afor its good speciality and reproducibility and easiness to judge.