Inhibition effect of genistein on proliferation of prostate carcinoma PC-3 cells

  Objective   To study the influence of Genistein on the growth of prostate carcinoma PC-3 cells in vitro.

  Methods   Prostate cancer cells were grown in DMEF 12 medium supplemented with 10% bovine serum and 5 ml 10 000 U/ml of penicillin/streptomycin in an incubator maintained at 5% CO₂ and 37℃.Using cell culture tests(MTT test, index of cellular mitosis, Cloning for mation and ³H-T dR incorporation test), We examined the growth of PC-3 cells at four concentrations genistein 10, 20, 40, 80 μmol/L with a negative control(anhydrous alcohol and DMSO v/v).

  Results   Genistein could inhibied the growth of PC-3 cells.After treatment with various geinistein concentrations mnentioned above for 7 days, the inhibition rates were 27.7%, 58.8%, 72.3% and 84.4% respectively.With the increase of genistein concentrations, Mitotic index and Cloning formations were decreased in PC-3 cells and the quantity of ³H-T dR incorporation was significantly decreased as compared with the negative control(P < 0.05).

  Conclusion   Genistein can significantly inhibit the growth of PC-3 cells and it is probably one of the preventive mechanisms of genistein in prostate cancer.