Relationship between expression of IAPs and infection of HPV_16/18 in uterine cervical carcinogenesis

  Objective   To investigate relationship between expression of inhibitors of apoptosis proteins(IAPs), such as Bcl-2 and Survivn, and HPV_16/18 infection in uterine cervical carcinogenesis.

  Methods   Formalin-fixed, paraffin-embedded samples from cervical carcinomas, cervical intraepithelial neoplasia(CIN)and normal cevical tissues were studied.Using streptavidin-biotin roxidase(S-P)immunohistochemical technique, the authors examined the expression of Bcl-2 and Survivin in these samples.The number of apoptosis cells was assessed in situ by TdT-mediated dUT P-biotin end labeling(TUNEL) method.The infection of HPV type 16, 18 DNA were determined by PCR.

  Results   There were significant differences for Bcl-2 and Survivn and AI were corrlated with tumor grades, clinical stages and lymph node metastasis.The positive rate of HPV_16/18 was significant difference between cervical carcinomas, CIN and normal tissues and was not asso ciated with tumor grades, clinical stages and lymph node metastasis.The infection of HPV_16/18 was associated with the expression of Bcl-2, Survivin and AI, respectively.

  Conclusion   The abnormal expression of Bcl-2 and Surviv in are associated with the infection of HPV_16/18.They possibly become useful indexes for the primary screening and prognosis of cervical carcinomas.