Effect of glutathione modified magnetite on growth of human lung adenocareinoma cell line SPC-A1

  Objective   To study the effect of GSSG modified superpar amagnetic particles on the growth of human lung adenocarcinoma cell line SPC-A1 in vitro.

  Methods   Human lung adenocar cinoma cell was cultured in the medium containing GSSG modified magnetic particles, the change of the cell morphology, was observed through inverted microscopy, the location of GSSG modified magnetic particles in the cytoplasm was observed by transmission electr on microscopy.Cell viability was determined by MTT reaction.LDH release level was measured by LDH detection kit.Cell growth curve was measured by using haemacy tometer after cell took up magnetic particles.

  Results   The morphology of cell did not change obviously after being cultured with magnetic particles.GSSG modified magnetic particles were located in the cytoplasm after cell subculture six generation.Culturing with three different concentrations of magnetic particles for different time, cell viability was more than 90 percent, LDH release level did not change obviously comparing with the control cell.Cell growth curve did not change greatly after cell uptake magnetic particles.

  Conclusion   At certain concentration range(0~1.5 mg/ml), GSSG modified magnetic particles have no obvious effects on the morphology, viability, subculture and growth curve of cell, magnetic particles have fine biocompatibility.Transmission electron microscopy images show magnetic particles can stay in the cytoplasm after cell subculture several generations, and magnetic particles have high reservation in the cell.GSSG modified magnetic particles have many potential applications in biomedicine.