Effects of RNA interference on Bax gene on 293 cell apoptosis induced by cadmium

  Objective   To study the effects of Bax in 293 cell apoptosis induced by cadmium chloride with RNAi.

  Methods   Cells were incubated with CdCl₂ after siRNA transfection.The expressions of Bax were detected by RT-PCR and western blot, the rates of apoptosis were measured by flow cytometry(FCM)and the activities of cells were measured by MTT.

  Results   The apoptosis rates of 293 cells treated with 30LM CdCl₂ were higher than those of the control group accompanied by increased expressions of Bax.siRNA-Bax could inhibit those changes but negative control and transfection agent couldn't.

  Conclusion   Bax is a key role during cadmium chloride induced 293 cells apoptosis.