Detection of Enterohemorrhagic escherichia coli O157: H7 in food with PCR assay

XING Lixin; SUN Wuchang; LIU Guihua

doi:10.11847/zgggws2006-22-12-55

XING Lixin, SUN Wuchang, LIU Guihua, . Detection of Enterohemorrhagic escherichia coli O157: H7 in food with PCR assay[J]. Chinese Journal of Public Health, 2006, 22(12): 1504-1505. DOI: 10.11847/zgggws2006-22-12-55

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Detection of Enterohemorrhagic escherichia coli O₁₅₇: H₇ in food with PCR assay

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Abstract

Objective To achieve rapid detection of Enterohemorrhagic escherichia coli O ₁₅₇: H₇ in food was achieved by using multiplex PCR assay.
Methods The virulence genes of EHEC: coli O₁₅₇: H₇ like toxin STX, rfbE, flic were chosen as the terget genes and amplified in multiplex PCR assays.
Results The assay was designed to amplify the 228, 378 and 709 bp regions of cornesponding virulence three genes And onl EH EC: coli O₁₅₇: H₇ was amplified.No specific amplication product was producied from other strains.
Conclusion
Conclusion PCR method is more concise, rapid, sensitive and more specific than conventional culture method.It is proved to be a new method for rapid detection EHEC: coli O₁₅₇: H₇ in food.

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Detection of Enterohemorrhagic escherichia coli O157: H7 in food with PCR assay

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Detection of Enterohemorrhagic escherichia coli O₁₅₇: H₇ in food with PCR assay