Effects of manganese chloride on exponent of birth and quantity of seminiferous epithelium cell of mice

Objective To study the effects of manganese chloride on reproductive function of male mice,and explore the dose effects,and time-effects.Methods The mice were randomly divided into three treatment groups with manganese chloride(7.5,15.0,30.0 mg/kg)and 1 control group with NS.To observe the weight coefficient of testis after 5 different periods(3,7,14,28,56d)i.p.administration MnCl₂(7.5,15.0,30.0mg/kg),the reproductive ability of male mice,changes of cross section area of seminiferous tubule and quantity of seminiferous epithelium cell were investigated after 56 d of manganese exposure.Results After 56day manganese exposure,the weight coefficient of testis in 15,30 mg/kg groups had distinctive difference as compared with control group(p<0.05,p<0.01);the exponent of birth and the radio of dead fetus in 30 mg/kg group had significant difference as compared with control group(p<0.05,p<0.05);and the average of live fetus and ratio of nidation in 3 manganese exposure groups had distinctive difference as compared with control group(p<0.01);cross section area of seminiferous tubule and quantity of seminiferous epithelium cell as compared with control group;the difference had statistical significance(p<0.01).Conclusion The reproductive ability of male mice,it was the most serious in 30 mg/kg group after 56-day manganese exposure.