Separation of micro molecular protein in human serum with improved Tricine-SDS-PAGE system

ObjectiveTo separate less abundant proteins less than 10 000 Da with an effective Tricine-SDS-PAGE system.MethodsThe human serum albumin was removed by Hitrap Blue column,and serum was dealed with U9.Then gel was stained by the improved method(0.1% carbinol,0.5% TCA,0.1% Coomassie briliant blue G250).ResultsThe discovery and detection of less abundant proteins less that 10 000 Da were improved obviously by removal of human serum albumin,serum treated with U9 and improvement of staining.ConclusionThe improved Tricine-SDS-PAGE system is not only effective to resolve these less abundant proteins less than 10 000 Da,but also favourable to recover these proteins from gel.