Study on gastrointestinal function in obesity prone rats and obesity resistant rats induced by diet

ObjectiveTo explore the differences of nutrients digested and absorptive as well as gastric empting,and intestinal transit in obesity prone(OP)rats and obesity resistant(OR)rats induced by high-fat diet.MethodsMale Sprague Dawley(SD)rats were divided into OP rats and OR rats fed with high fat diet for 8 weeks.Then a three-day metabolism experiment was carried,and feces and urine were collected to measure the fat and protein.Phenol red administration was used to measure the rate of gastric empting and inkadministration was used to measure the rate of intestinal transit.ResultsObesity prone(OP)rats used more protein and fat than obesity resistant(OR)rats.The gastric empting rate had no significant difference in obesity prone(OP)rats and obesity resistant(OR)rats.The rate of intestinal transit in obesity prone(OP)rats was higher than that in obesity resistant(OR)rats.ConclusionWith the high fat diet,one of the reasons that result in obesity prone(OP)rats and obesity resistant(OR)rats is the difference of nutrients digested and absorptive and gastrointestinal motor.