Oxidative damage of mitochondria and micriosome in testis cells induced by nickel sulfate in rats

ObjectiveTo explore the mitochondria and microsome of testis cells caused by nickel sulfate in rate.MethodsThirty-two male Wistar rats with sexual maturation were divided into four groups randomly,and three groups of rats were administrated intraper itoneally with nickel sulfate daily at doses 1.25,2.50,5.00 mg/kgrespectively for 30 days,but the control group was administrated with saline in same volume according to the rats' weight.The mitochondria and microsome of test is cells were prepared by centrifugation technique to detect the levels of lipid peroxidation(LPO),total antioxide capacity (T-AOC),reactive oxygen species(ROS)in spectrophotometry.ResultsThe organ coefficient of testis exposed to NiSO₄ had no change compared wth the control group(P<0.05).The LPO and ROS contents in testis of exposure groups were higher than that of control group(P>0.05),but the T-AOC levels of the exposure groups were decreased compared with the control group(P<0.01).ConclusionThe rat's testis damage is related to the enhancement of oxidative stress of mitochondria and microsome caused by nickel sulfate.