Influence of green tea polyphenols on caveolin-1 expression in bovine endothelial cells

Objective To investigate the influence of green tea polyphenols(GTPs)on caveolin-1(Cav-1)expression in bovine endothelial cells in the level of cell and molecular initially.Methods In vitro,primary cultured bovine artery endothelial cells(BAECs)were treated with 4 μg/ml GTPs for 0,4,8,12,16 and 24 hours,respectively.After protein and RNA extracted from treated cells,the Cav-1 protein and mRNA levels were detected by Westem Blot and RT-PCR methods.Results After BAECs co-incubated with 4 μg/ml GTPs for 4 hours,the expression of Cav-1 protein and mRNA decreased.The down-regulation effect of GTPs can maintain 4 hours and protein and mRN A expression of Cav-1 regained to normal level after 8 hours.Conclusion GTPs can decrease the protein and mR NA expression of Cav-1 in endo thelial cells,and the influence of GTPs may be chronergy.It may be the molecular mechanism of tea amelioration endo thelial function and prevention CVD.