Protective effect of taurine against DNA damage of human derived fetal hepatocytes induced by Benzo(a) Pyrene

Objective To investigate the protective effect of taurine on DNA damages of human derived fetal hepatocytes induced by B(a)P.Methods Human derived fetal hepatocytes(L-02 cells)were randomized into 5 groups:(Ⅰ)blank control group;(Ⅱ)solvent control group;(Ⅲ)B(a)P group explored to B(a)P at the concentration of 25μmol/L,in culture medium for 2 hours:(IV)Taurine group explored to taurine at the concentration of 0.5,1.0,2.0,4.0 mmol/L in culture medium for 24 hours:(V)Taurine preventive group explored to taurine at 4 different concentration in culture medium for 24 hours and treated further for 2 hours by adding 25μmol/L B(a)P into the culture media.Single cell gel etectrophoresis (SCGE)were used to detect DNA damage.The concentration of MDA,GSH,AST,ALT in the supernatants of culture media were tested by spectrophotometric method to reflect the oxidative damage level of cell.Results (1)Only a low concentration of B(a)P could induce DNA damage of L-02 cells and cause the concentration of MDA,AST,ALT in the supernatant increased and GSH decreased.(2)Pretreatment with taurine could antagonize significantly the toxicity of L-02 cells induced by B(a)P.It could reduce the MDA,AST,ALT level and increase GSH content in cells in dose-effect manner.(3)The DNA damage was positive correlation with MDA,AST and ALT levels.However,it was negative correlation with GSH level.Conclusion Oxidative damage might be a important mechanism with regard to DNA damage of human derived fetal hepatocytes induced by B(a)P;Taurine could play a protective role in hepatic cell damage by means of ant-oxidation effect.