Expression and identification of novel genes differentially expressed in diabetic rats supplemented with zinc

ObjectiveTo express and identify novel gene fragments differentially expressed from diabetic rats supplemented with zinc.MethodscDNA fragments from former research project were cloned,sequenced and BLASTn analysed.The RT-PCRof the six novel genes were made to observe the expression changes in liver of various groups.ResultsZn-2, Zn-6,Zn-10,Zn-14,Zn-19,and Zn-20 cDNA were shown to be the novel gene fragments for no matched gene with them in genbank.The expression level of 4 of them,in both DM group and DM+Zn group were obviously lower than those of NC group.The expression level of DM+Zn group was higher than those of DM group(P<0.05).They were registered to genbank with the numbers of AY 952968,AY952970,DQ 351838,DQ351839,respectively.ConclusionThe four novel genes differentially expressed in diabetic rats supplemented with zinc were cloned,and were successfully submitted to genbank.