Evaluation of different dispersal liquid for nanoparticles

Objective In order to screen effective dispersal liquid of nano-particles with low toxicity.Methods By macroscopic observation,transmission electronmicroscope(TEM)syndro me and the determination of lung damage to rats,the dispersal effect of four dispersal liquid-0.9% physiologic saline,new born calf serum,priv ate made micro emulsion,1% new born calf serum albumin were evaluated.Results Nanocarbon particles combined with nano ZnO was uniform and stable in the new born calf serum,and was settled faster in the saline.The result of TEM sy ndrome showed that the nano particles was ag glomeration in the saline,the biggest particles diameter being 60 nm.The particles was distributed well in new born calf serum,the particles diameter being 20~40 nm.The particles per iphery was enveloped in micro emulsion with the diameter 100nm and the particle's diameter was not observed in serum albumin.Comparing the lung to xicity of the particles liquid,the concentrations of NO in bronchoalveolar lavage(BAL)increased significantly in micro-emulsion and saline groups with com centration of(146.41±7.02),(162.34±15.87)μmol/L separately.The concentrations of NO and total protein(TP)in BAL decreased significantly in calf serrum and albumin groups,N O(79.05±13.01),(11.45±4.91)μmol/L;TP(0.30±0.03),(0.20±0.01)g/L.The results of nano-particles toxicity to lung in rats showed that in nano ZnO groups the activity or concentration of GSH-PX,NO and TP increasing more significantly(128.02±68.11)U/(mg#prot),(144.34±50.88)μmol/L,(0.50±0.19)g/L.In nano carbon combined with ZnO groups TP(0.52±0.14)g/L,NO(154.08±15.41)μmol/ L,increased significantly than in control groups.Conclusion New born calf serum was a better dispersal liquid to nano particles,and injured relatively little to body and could reflect the to xicity of nanopartiles correctly.