Effect of acrylamide on proliferation and differentiation of rat spinal cord nerve cell in vitro

Objective To present experimental studies in which midbr ain nerve cell of rat spinal cor d were contaminated with acrylamide(ACR).Methods The primary culture of midbrain cell of new born rat spinal cord pruification was made after separated and digested.Acrylamide was added with doses of 0,150,300,450,600,750 g/ml ACR respectively.The cells culture was observed with methods of cytomor ghology and cell counting.The concentration rate were counted and the content of protein the MTT,malondialdehyde(MDA)and activity of superoxide dismutase(SOD)were determined.Results 150,300 g/ml of acrylamide had no inhibitory effect on the spinal cord neurons.The effect dev eloped when the concentration was up to 450,600,750 g/ml,and the inhibitory effect of acrylamide became more significant with the increase of the concentra tion.Conclusion The inhibition of acrylamide on rat spinal cord nerve cells might be associated with impact of protein synthetis and DNA.