A multiplex PCR method to identify salmonella enterica serovars Typhi and Paratyphi A

Objective To developa multiplex PCR method for identification of salmonella enterica serovars Typhi and Paratyphi A.Methods The PCR primers for O,H,and Viantigen genes,rfbE,rfbs,flic-a,and viaB,were designed and used for detecting 54 tested bacteria strains,including 47 Salmonella strains and 7 other bacterial strains.Results All the examined isolates of Salmonella serovars Typhi and Paratyphi A were accurately identified by this assay.Conclusion The multiplex PCR can identify Salmonella enterica serovars Typhi and Paraty phi A with high specificity.