Microwave irradiation induced microglia activated and JAKs phosphorylated in rathippocampus

Objective To deter mine the activation of microglia and the phosphorylation of Jaks,the upstream factors of JAK/STAT(janus activated kinase/signal transducers and activators of transcription)signaling pathway after microwave irradiation.Methods Rats were irradiated by 90 mw/cm² EMF for 20 min.The phosphor ylation of Jaks was deter mined by western blot at different time after mcirowave exposure.The activation of microglia was determined by immunochemistry.Results GSA-IB4 was upregulated in microglia,which indicated microglia was activated after microwave exposure.The phosphor ylation of Jak1,Jak2 and Jak3 in rathippocampus was upregulated after microwave exposure.The phosphorylation of Jak1 was upregulated after microwave exposure and peaked at 12 h.Jak2 peaked at 0 h after microwave exposure and sustained in a high level.Jak3 was slightly affected by microwave exposure.All the thr ee members of JAKs returned to normal at 72 h after microwave exposure.Conclusion Microglia cells was activated after microwave exposure.The phosphor ylation of Jaks was upregulated by microwave irradiation.JAK/STAT singnaling pathway was activated after microwave irradiation,which indicated that JAK/STAT signaling pathway may participate in microwave induced brain microglia activation