Effects of c-FLIP antisense oligodeoxynucleotides on growth and apoptosis of lung cancer cell line A549

ObjectiveTo study the effects of cellular Fas-associated death domain-like IL-1 beta converting enzyme inhibitory protein(c-FLIP)antisense oligodeox ynucleotides(ASODN)on the growth and apoptosis of lung cancer cell line A 549.MethodsSynthesized ASODN of targetc-FLIP was transfected to lung cancer cell line A 549 at different concentration.Control group,liposome group,non sense oligonucleotide(NSODN)group were set for comparison.Living cells were counted by trypanblue method to draw cellular growth curve.Immunohistochemistry(SP)technique was also used to observe the expression of c-FLIP and Ki-67 antigen in the transfected tumor cells.MTT and flow cytometric were used to analyze the cell growth inhibition rate and apoptosis rate.ResultsAfter treated with c-FLIP ASODN,the growth and caryocinesis of A549 cell were inhibited significantly,especially in 4 μg/ml ASODN group.With the increasing of ASODN concentration the expression of c-FLIP and Ki-67 antigen was fall-off.The apoptosis rate of A549 cells was increased.Statistically significant difference was found compared with the other groups(P<0.05).No statistically significant difference was found among the control group,liposome group and NSODN group(P>0.05).ConclusionThe c-FLIP ASODN could effectively inhibit the expression of c-FLIP gene and the growth of lung cancer cell line A549 and induce the apoptosis with a dose-effect relationship.It may be one of the promising medicine for antisense gene therapy in lung cancer.