Detection of staphylococcus aureus enterotoxin B in burn infection by double sandwich enzyme-linked immunosorbent assay

ObjectiveTo establish a two site antibody enzyme-linked immunosorbent assay(ELISA)for detecting SEBin burn infection patients.MethodsA monoclonal antibody to staphylococcus aureus B(SEB)was used as indicator antibody.The sensitivity of ELISA was improved by biotin binding system.ResultsCalibration curves for SEB were linear from 0.078~10.000 μg/L(r=0.9876).Data showed that SEB levels after the severe burn followed by staphylococcus aureus infection were significantly higher than those of normal controls and slight burn control group(P<0.001).ConclusionThe method is rapid with high sensitivity(0.078 μg/L)and could be used to detect SEB in hospital infection patients.