Cloning and sequence analysis of allergenic parvalbumin gene from red snapper(Lutianus argentimaculatus)

Objective To clone allergenic parvalbumin genes from red snapper.Methods All the known parvalbumin gene sequences from several fish species were compared by bioinfor matic method and the highly conserved domains were selected for degenerative primer designing.The RT-PCR and 3'-RACE methods were applied to clone the full-length parvalbumin cDNA from red snapper.Results Our data indicated that the full-leng th cDNA of red snapper parvalbumin containsed 608 bp nucleotides.It encodes a 109 amino acid protein with an estimated molecular weight of approx imately 11.5 kD and an isoelectric point of 4.35.The predicted pro tein sequence showed high homology with aller genic parvalbumin from many other fish species.Conclusion An allergenic par valbumin gene was cloned from red snapper(access number: EF591789).