Effect of TAD compound on apoptosis and cell cycle progression of lymphoma cells in vitro

Objective To observe the effect of 3,4,5-trihydrox ybenzoic acid dimmer(T AD)in different concentration on apoptosis and cell cycle prog ression of lymphoma EL-4 cells in v itro and ex plore its possible mechanism of tumorg rowth inhibition.Methods Survival rate was observed with M T T assay.The changes of apoptosis and cell cycle progression were measured with flowcy tometry.Results TAD of 3.125,6.25,12.5 and 25 Lg/ml could inhibit the proliferation of El-4 lymphoma cells in vitro.The survival rates of the cells treated with TAD were significantly lowerthan that of in the control(P<0.01,P<0.001).The apo ptotic percentag es of the cells treated with TAD increased signficantly as compared with those in the control(P<0.01,P<0.001).And the percentages of G0/G1 phase cells treated wit h TA D were significantly hig herthan those of in the control(P<0.05,P<0.01,P<0.001).While the percentages of Sphase cells treated with TAD were significantly lowerthan those in the control(P<0.05,P<0.01,P<0.001).The percentages of G2 phase cells did not change significantly.Conclusion The inhibiting effect of TAD on tumorgrowth could be due to cell apoptosis caused by G0/G1 phase arrest.