Purification of the antimicrobial peptides with anti-Toxoplasma gondii effect isolated from Musca domestica larvae

Objective To isolate and purify the antimicrobial peptides with anti-toxoplasm a gondii activity isolated from the haemlymph of M usca domestica larvae.Methods The antimicrobial peptides of Musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.Then the antimicrobial peptides with anti-toxoplasma activity were sieved by M TT colorimetric method and haemacytometry.Results It was found that two antimicrobial peptides had anti-toxoplasma activity through Resource Scationic column chromatography and Superdex G75 gel column chromatography.Conclusion The two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of M usca domestica larvae had different anti-toxoplasma effect.