Cloning and application of CMV gp52 protein

Objective To construct the recombinant plasmid and engineering bacteria for expression of CMV gp52 protein and to use the expressed product for detection of CMV specific antibody.Methods Using PCR technology,recombinant CMV gp52 was cloned and expressed to establish IgM capture ELISA,and the product was detected for its antigenicity and practicalit y.Results Through expression and purification,the purity of gp52 was over 95%.IgM capture ELISA established by gp52-HRP was used to detect 35 CMV-positive serums and 35 CMV-negative serums,the positive detection rate was 97.1% and negative detection rate was 100% by capture ELISA with no significant difference compared with SORIN company'kit(P>0.05).gp52 antigen can response to the one CMV-positive serum(1:16 dilution),so the gp52 had better antigenicity.Conclusion CMV gp52 protein efficiently expressed had specific antigenicity,and can be used for establishing IgM capture ELISA to detect cytomegalovirus antibodies.