Isolation, identification and purification of allergens in cashew nut

Objective To isolate,identify and purify the allergens in cashewnut,anacard lun occidentale L.Methods The total protein of the cashew nutwas iso lated by SDS-PAGE.The allergens of cashew nutwere identified by Westernblotting.To purify the allergens,ion exchange chromatography was employed.Results There were tenprotein bands visible on the SDS-PAGE gel.The allergens with molecular mass of 21kD and 11kD were identified by Western-blotting.The 21kD and 11kD allergens of cashew nutwere purified by ion exchange chromatography.Conclusion The allergens of cashewnut were isolated and identified.And the allergens of cashewnut were purified prmiarily.