Effect of static magnetic field on proliferation and differentiation of rat kidney epithelial cells in vitro

Objective To study the effect of static magnetic field(SM F)on differentiation of kidney epithelial cells in rat.Methods The prmiary culture of new born rat kidney epithelial cells was exposed to SMF at the intensity of 5,10,20,30,40,50 and 60 mT.The cell culture was observed with methods of cytomorghology and cell counting.And the content of protein,the MTT,malondia ldehyde(MDA)and activity of superoxide dismutase(SOD)were determ inded.Results The SMF inhibited differentiation of the rat kidney epithelial cells and a ffected cell proliferation.In the expermiental groups with 40,50,60 mT the relative content of protein decreased and the level of MDA and the activity of SOD decreased obviously(P<0.05).Conclusion The inhibition effect of SMF on rat kidney epithelial cell smight be a ssociated with protein synthetis and lipid peroxidation.