Effects of sulforaphane on expressions of ICAM-1 and VCAM-1 mRNA induced by LPS and nuclear translocation of NF-κB in vascular endothelial cell

Objective To study the effects of sulforaphane on the expressions of ICAM-1 and VCAM-1 mRNA induced by lipopoly saccharide(LPS)in vascular endothelial cell ECV 304 and its tran scriptional mechanism.Methods The levels of mRNA of ICAM-1 and VCAM-1 were determined by quantitative real-tmie PCR and nuclear translocation of NF-κB was measured by immunofluorescence hybridization.Results Treated with 5,10,20 mol/L sulforaphane followed by LPS,the expressions of ICAM-1 mRNA in ECV 304 were deminished to 2.3,1.5 and 1.1 fold of the level of the control,respectively.Compared with LPS,sulforaphane significantly inhibited the expression of ICAM-1 mRNA in dose-dependent manner(P<0.05).5 mol/L sulforaphane down-regulated expression of VCAM-1 mRNA induced by LPS to 1.4 fold,while 10,20 mol/L sulforaphane completely elmiinated the stmiulation and the level of VCAM-1 mRNA was only 0.4 fold of the control(P<0.05).The weakness of the strength of green fluore scence in nuclear indicated that 10 mol/L sulforaphane could block the translocation of NF-κB.Conclusion Sulforaphane down-regulates the expression of ICAM-1 and VCAM-1 mRNA through blocking the translocation of NF-κB into nucleus.