Effects of combined use of DMSA with calcium and ascorbic acid on fetal development in lead intoxicated pregnant mice

ObjectiveTo study the joint effects of DMSA and nutrients on lead into xicated pregnantmice and the development of their offspring.MethodsFele healthy mice were exposed to 0.1% lead acetate in drinking water for 4 weeks,and then randomly divided in to lead control group,DNSA group and therapeutic alliance group(DMSA+Ca+Vit C).All the felale mice were mated to male by the proportion of 2:1.After the female mice had a pregnancy for four days, the treatment were supplied by gastro gavage every other day during the pregnancy.At the end of the experiment, the blood lead levels of female mice were measured.One to three mouse offspring were taken to measure the Hb content and the lead level in the liver and bone.And then,physio logical developments of the offspring mice were observed and calculated.ResultsThe blood lead levels of female mice in DMDA group and therapeutic alliance group were lower than that of lead control group(P<0.05).The Hb level of the offspring in DMSA group was the lowest and significantly lower than that of blank control group(P<0.05).The lead levels in the liver and bone of the offspring in DMSA group were higher than that of lead control group and the rapeutic alliance group(P<0.05).The time of pinna detachment, tooth growth development of the offspring in DMSA group were later than those of lead control group(P<0.05).The time of tooth growth of the offspring in therapeutic alliance group was later than those of lead control group(P<0.05).ConclusionThe physiological development of the offspring of the female mice treated with DMSA after lead exposure were delayed because DMSA increased the lead transmitted to the offspring through placenta.