NQO1,HSP10 and 2-Cys Prxs expression in umbilical cord blood mononuclear cells induced by hydroquinone

Objective To explore the effect of low dose hydroquinone(HQ)pretreahnent on the cell viability and the expression of NQ01,HSP10 and 2-Cys Prxs in umbilical cord blood mononuclear cells (UCBMC).Methods Mononuclear cell from cord blood were isolated and Annexin V/PI staining assay was applied to evaluate the effect of low level HQ on human hematopoietic system cells Real tine PCR was perfouned to deteunine the expression of NQ01,HSP10 and 2-Cys Prxs.Results The results ofAnnexin V/PI staining assay showed that the UCBMC weremore tolerable to 250μmol/L HQ after pretreated with 10μmol/L HQ at cell survival level (44.92±25.10 vs 39.37±20.98).The results of real-tine PCR showed that compared with the control group,the expression of HSP10,Prx3 and Prx4 significantly increased by 2.22± 1.03,3.44±1.07 and 2.97±0.79-folds,respectively,in high dose group.The Prxl was increased by 1.08±0.29-folds in adaptive group.The expression of NQO1 was increased by 3.44±1.07 and 3.57±1.31-folds,respectively,in high and adaptive group.The expression of Prx4 in high dose group was significantly increased by 2.41±0.31-folds compared with that of the control and with a significant difference from that of in adaptive group.Conclusion Prxl,Prx3,prx4,and Hsp10 may take part in the adaptive response of UCBMC induced by hydroquinone.