Association between rDNA-ITS polymorpholism and metronidazole resistance in Trichomonas vaginalis

Objective To study there lationship between the polymorpholism of rDNA-ITS and the resistance to metronidazole in Trichomonas vaginalis.Methods Thirty-nine clinical isolated strains of Trichomonas vaginalis were detected for resistance to metronidazole in aerobic condition with 96-wells plates and rDNA-ITS were amplified and sequenced.Results Twenty strains were metronidazole susceptible and 19 strains were metronidazole resistant.A285bp rDNA-ITS and 5.8S fragment were amplified and sequenced.Only one point mutation(C66T)was detected by sequence a lignment with MegA lign of DNA Star softw are.A total of 8 stra ins were detected in which 6 strains were from metronidazole-susceptible group and the other 2 from the metronidazole resistant group.There was no statistics difference between susceptible and resistant strains with Fishe rexact test(P>0.05,χ²=0.108).Conclusion There is no obvious association between C66T mutation of rDNA-ITS and resistance to metronidazole in T.vaginalis.