Inhibitive effect of vitamin C on embryonic development toxicity induced by formaldehyde in mice

ObjectiveTo observe the inhibitive effect of vitamin C(VC)on embryonic development toxicity induced by formaldehyde in mice.MethodsKunming mice embryos were incubated together with formaldehyde(9,27,and 81μmol/L)and VC(100μg/mL),respectively,with whole embryo culture method.The development of mice embryos was then a ssessed.ResultsWhen the concentration of formaldehyde was 9μmol/L,the increa ses of head lengh (1.66±0.21mm),cranialarm length(3.48±0.40mm),glutathione(GSH)(47.70±2.12mg/g prot)and malona ldehyde (MDA)(4.27±0.62nmol/mg·prot)were significantly different(P<0.05)from those of the control group.When the concentration was 27μmol/L,the increases of embryo visceral yolk sac(VYS)diameter(4.35±0.65mm),head lengh (1.48±0.30 mm),cranial arm length(3.19±0.43mm),number of arthromera pairs(25.2±1.2)and GSH (40.25±4.38mg/g·prot)and the decrease of MDA(6.29±0.47nmol/mg·prot)were observed with significant differences(P<0.05)copared with tha t of the control group.VC could improve the indexes but there was no significant difference between the control group and the VC group(P>0.05).The toxic injury induced by form aldehyde(81μmol/L) could not be improved with VC significantly.ConclusionV Cinhibits embryonic development to xicity induced by certain dose of formaldehyde.