Differential gene expression profile induced by ionizing radiation in thymic lymphoma of mice

ObjectiveTo investigate the differences of gene expression induced by ionizing radiation in thymiclym-phoma of mice.MethodsBALB/c mice were subjected to whole body irradiation with 1.75Gy X-ray once a week for 4 weeks to induce thymiclym phoma.The difference of gene expression in thymic cell of mice was detected by Mouse WG-6v2.0 Expression BeadChip.The real-time PCR was used to identify important genes.ResultsThe chip depicted 3 063 genes with differential gene expressions including 1 591 up-regulation and 1 472 down-regulation genes.The genes related to 145 pathways of KEGG database,76 pathways of Bio Carta database and 218 pathways of Gen MAPP database.The results of real-time PCR showed that the mRNA expression level of Bcl11b and Ikzf1 genes tended to be down-regulated.ConclusionThe ionizing radiation may induce differential gene expression profile in thymic lymphoma of mice,and the process may involve many pathways.