Apoptosis and expressions of c-jun, c-fos in rat hippocampal cells in vitro with continuous hypoxia

ObjectiveTo observe the cell apoptosis and the expressions of c-jun,c-fos in neonatal SD rat hippocampal cells with continuous hypoxia in vitro.MethodsAfter ten-days primary culture,the hippocampal cells were exposed to 90% N₂,5% O₂,5% CO₂ for 2,6,12,and 24 hours.The apoptosis of the cells was identified by Hoechst 33258 staining and the expressions of c-jun,c-fos was analyzed with western-blot.ResultsWith the extension period of hypoxia,the number of apoptotic cells was significantly increased from 23.79±3.43% to 74.36±5.58%.Western-blot assay showed the expressions of c-jun,c-fos increased at 2 hr,and reached the highest at 6 hr and then gradually decreased,but were still higher than those of the control at 24 hr.ConclusionContinuous hypoxia can induce the expression of c-jun,c-fos in hippocampal cells in vitro,which might be related to the cell apoptosis and the neuron protective mechanism.