Acute oral toxicity and mutagenicity of powder extracted from larvae of Musca domestica in mice

ObjectiveTo evaluate acute oral toxicity and mutagenicity of powder extracted from larvae of Musca domestica(PLMD)for safety assessment.MethodsLarvaes of Musca domestica were smashed in the solution with ultrasound.The solution was vacuum-dried into powder and the shells was processed into chitosan.The powder and chitosan were mixed with PLMD at the ratio of 10 vs 1.Acute toxicity test,mouse bone marrow cell micronucleus test and mouse sperm abnormality test were conducted.ResultsThe oral maximum tolerated dose of PLMD was more than 33.0 g/kg body weight for mice.The polychromatic erythrocyte micronucleus rate of the male mice treated with PLMD at the doses of 2.8,5.5,and 11.0 g/kg·bw was 0.12%,0.14%,and 0.14,and those of female mice was 2.3%,1.6%,and 2.1%,respectively.The differences were of no statistical significance.ConclusionThe PLMD has no acute oral toxicity and mutagenicity effects on mice.