Expression of Wnt5a in renal tissue of diabetic rats and its significance

Objective To explore the expression of Wnt5a in renal tissue of diabetic rats.Methods The diabetic rats induced by streptozotoc in(STZ) were randomly divided into 2, 4, 8, 12, 16, and 24 weeks groups and each group was set a case matched control group Blood glucose, 24 hour urine protein, serum creatinine(Scr) of the rats were measured.The prote in and mRNA expression of Wnt5a and fibronectin(FN) in renal cortex were detected with immunoh istochemical staining and reverse tran scriptase-PCR (RT-PCR).Western blotting was employed to detect the expression of Wnt5a protein.Results The Wnt5a prote in could not be detected by immunohisto chemical staining in normal renal tissues.Compared to the control group, the expression of Wnt5a prote in were highly increased by 65.5%, 72.2%, 60%, 68.8%, 68.8%, and 63.0% (P<0.01) by western blotting in diabetic rats.The mRNA of Wnt5a and FN were significantly up regulated by 50.9%, 50.0%, 35.7%, 50.9%, 55.0%, and 54.2% and 35.5%, 64.6%, 34.1%, 61.8%, 66.7%, and 64.6% (P<0.01) in the diabetic rats compared with those of the controls.The expression of Wnt5a mRNA was positively correlated with FN in diabetic rats (r=0.714, P<0.01).Conclusion The findings suggest that Wnt5a gene and prote in expression are up-regulated in the kidney of diabetic rats,which may involve in the pathogenesis of diabetic nephropathy.