Potassium iodide method：a qiuck way of genomic DNA extraction from human blood clotting

ObjectiveTo establish a simple,quick and economical method for genomic DNA extraction from human blood clotting compared with traditional phenol-chloroform method.MethodsDouble distilled water was used to lyse red blood cells(RBC)and saturated potassium iodide to lyse white blood cells(WBC)and its nuclear membrane.Chloroform and isoamyl alcohol was used to precipitate proteins and the residues of cells.The the genomic DNA was precipitated by isopropanol and ethanol.ResultsThe average quantity of genomic DNA extracted was 46.4±8.8mg/L and the ratio of A₂₆₀/A₂₈₀ was 1.79±0.23.Furthermore,no statistical signifcance was observed when compared with phenol-chloroform method.The electrophoretic bands of genomic DNA and PCR amplification products were distinct,and the extracted genomic DNA reached the standard for molecular biological experiment.ConclusionThe postassium iodide method is a simple, quick,economical and efficient way for genomic DNA extraction from human blood clotting,and could be used in large genomics study.