Effects of celery on Bcl-2 and Bax in spermatogenic cells in male mice

ObjectiveTo explore the effects of celery on Bcl-2 and Bax in spermatogenic cells in male mice.MethodsTotally 133 adult male Kunming mice of SPF degree were randomly divided into normal saline,low,moderate,and high dose group according to the body weight and subdivided into 7,14,and 28 days group for each of the 4 groups.All mice were given intragastric administration of celery juice every day.The experimental groups were treated with 0.3 mililiter of celery juice at different concentrations and the control group with 0.3 mililiter of normal saline.All mice were killed and the testis was collected at the end of the treatment.The contents of Bcl-2 and Bax in spermatogenic cells were determined with streptavidin-biotin complex(SABC)method.ResultsThe average values of optical density of Bax in every dose group were significantly different from that of control group(P<0.05)7 days after the treatment.The average values of optical density of Bcl-2 in every dose group were significantly different from that of control group(P<0.05)28 days after the treatment and that of the low-dose group was significantly different from those of the moderate and high dose group(P<0.05).ConclusionPro-apoptotic role of celery was not observed.Bcl-2 has an inhibitive effect on apoptosis of spermatogenic cells.