Protective effects of resveratrol on renal oxidative injury in rats

Objective To examine the protective role of resveratrol on lipid oxidation which may cause kidney damage in rats with high glucose and high-fat diet.Methods The rats were divided into normal diet control group,high glucose and high-fat diet group,low,moderate and high dose resveratrol groups.All rats were killed after 13 weeks.Serum glucose,total cholesterol,and triglyceride levels were determined.Total antioxidant capacity (T-AOC),total superoxide dismutase (T-SOD),glutathione peroxidase(GSH-Px) activity,and malondialdehyde(MDA) content were measured in renal tissue extracts and renal peroxisome proliferator-activated receptor alpha(PPARα),mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase(HMGCS2) mRNA expression were determined.Results Body weight (453.00±19.54 g),renal MDA (6.11±1.42 nmol/mg·Pro) content increased significantly in high glucose and high-fat diet group compared with normal diet control group,w hile decreased in resveratrol co-treated groups compared with high glucose and high-fat diet group.The renal T-AOC(0.27±0.03 U/mg·Por),T-SOD(2.43±0.07 U/mg·Pro) levels of high glucose and high-fat diet group were significantly lower than those of the normal diet control group.Compared with those of the high glucose and fat diet group,renal T-AOC,T-SOD,and GSH-Px levels decreased in resveratrol co-treated group.Compared with normal diet control group,lower renal PPARα and HMGCS2 mRNA expression levels were observed in high glucose and fat diet rats (P=0.012,0.045),while enhanced expression of renal PPARα and HMGCS2 mRNA were observed in the rats co-treated with resveratrol.Conclusion High glucose and high-fat diet can cause significant oxidative stress in rat kidney,which may be involved in the occurrence of renal damage.Resveratrol has protective effect on the high glucose and high-fat dietinduced oxidative renal injury in rats.