Detection of Shigella with helicase-dependent isothermal DNA amplification technology

Objective To establishe a new rapid method to detect Shigella based on helicase-dependent isothermal DNA amplification(HDA).Methods A highly specific set of primers was synthesized to target ipaH gene of Shigella and then HDA condition and the reaction system were optimized simultaneously.Specificity and sensitivity of the method w ere evaluated.Results The results of all three strains of Shigella were positive,and the othes were negative.The sensitivity was 5.1 × 10³ cfu/mL,which was similar to the result of PCR method.Conclusion Detecting Shigella with HDA is specific and sensitive as PCR method and has lower instrumental requirement.