Construction and identification of eukaryotic expression vector of MiR-29c

Objective To evaluate the effect of the intervention on knowledge of nutrition and health in puerperium among late-pregnant women.Methods Totally 640 late-pregnant women taking antepartum examination in No.8 Qingdao People's Hospital were randomly selected and divided into intervention group and control group.The women of intervention group accepted an education on nutrition and health knowledge.The two groups were followed up.Results Eventually 420 women completed the follow-up (213 in the intervention group,207 in the control group).The aw areness rate of nutritional and health know ledge of the intervention group was significantly higher than that in the control group.Women in the intervention group had more intakes of potato (105.87±68.85 g),fish (153.52±76.47 g),soy products (125.35 ±72.62 g),green leafy vegetables(168.78 ±100.81 g),nuts(40.14 ±41.73 g),dietary fiber(14.11 ±5.14 g),niacin(19.07±6.3 mg),calcium(782.57±294.87 mg),and vitamin C (131.48±76.00 mg) and had a higher score of health behavior(13.03±3.54) than those in control group.Women in the intervention group had less intake of egg (111.34±35.54 g),cholesterol(1494.70±650.18 g) than those in control group(t=1.99,4.30,P< 0.05 or P< 0.01),and had lower rate of disease(51.2%) than that in the control group(χ 2=21.12,P< 0.01).Conclusion The results show that specific nutritional health education could improve the know ledge level of puerperal women and promote healthy dietary and life behavior and reduce puerperal disorders.