Expression of Th1/Th2 cytokines in serum and maternal-fetal interface among recurrent spontaneous abortion women caused by Toxoplasma gondii infection

ObjectiveTo detect the expression of T helper-1 type(Th1)/T helper-2 type(Th2)cytokine in serum and maternal-fetal interface among recurrent spontaneous abortion(RSA)women caused by Toxoplasma gondii infection, and to study their clinical significance.MethodsThe special antibodies lgG,IgM,interleukin-2(IL-2),interleukin-4(IL4),interleukin-10(IL-10),and interferon gamma(IFN-γ)concentrations in serum or maternal-fetal interface of 79 RSA women were measured with enzyme-linked immunosorbent assay(ELISA)and 45 normal pregnant women in the first trimester about to abort artificially were assigned as controls.Results The positive rate(27.85%)of lgG in serum of RSA group was significantly higher than that of normal control group(11.11%,χ²=4.710;P<0.01),and the positive rate(10, 12.66%)of IgM in serum of RSA group was significantly higher than that of normal control group(4.44%,χ²=2.113;P<0.05).The IL-2 and IFN-γconcentrations in serum and maternal-fetal interface of new RSA patients were significantly higher than infected women in the control group,but the IL-4 and IL-10 concentrations were significantly lower(t=2.812-3.686;all P<0.01).ConclusionThe Th1 cytokines were expressed at a high level in serum and maternal-fetal interface of RSA and it could be one of the important factors for RSA.