Extraction of phytoestrogen lignan

ObjectiveTo explore a laboratory method for the extraction of secoisolariciresinol diglucoside(SDG)from flaxseed.MethodsIn the extraction of SDG,all factors were tested,including different extraction methods,the concentration of extract solution,the extraction time,and different alkali hydrolysis methods.Flaxseed SDG was detected by high performance liquid chromatography(HPLC).Results The SDG extraction is the most efficient with reflux extraction method.The best solution is 50% acetone,and the extraction time is 2 hours.The alkali extraction method is suitable with Ca(OH)₂-H₃PO₄ system.The purity of crude SDG was 32.48%.ConclusionMore SDG could be extracted in short timeusing the method established.