Effects of new targets of CDK2 RNA interference on proliferation of SHG44 cells

ObjectiveTo construct four new eukaryotic expression vectors of RNA interference specific for cyclin-dependent kinase-2 (CDK2) and transfect the vectors into SHG44 cells for the detection of vectors with strong interferential effect. MethodsFour new eukaryotic expression vectors of RNA interference specific for CDK2 were constructed.The human glioma SHG44 cell line was transfected with the four new vectors.The mRNA contents of CDK2 were detected using reverse transcriptase-PCR (RT-PCR).The change in proliferation of SHG44 cells was assayed. ResultsThe new vectors ith new targets of eukaryotic expression of RNA interference specific for CDK2 were constructed (P^CDK2-1,P^CDK2-2,P^CDK2-3,P^CDK2-4).CDK2 small interfering RNA (siRNA) could suppress expression of mRNA and P^CDK2-1.siRNA could inhibit the proliferation of SHG44 cell line. ConclusionThe proliferation of human SHG44 cell line could be significantly inhibited after the transfection with new eukaryotic expression vectors of CDK2 siRNA.