Association of CYP1A1 and CYP2E1 gene polymorphism with risk of arsenism caused by coal-burning

ObjectiveTo detect the distribution of CYP1A1 and CYP2E1 gene polymorphisms among patients with coal burning-induced arsenism and to explore the relationship between the risk of coal burning-induced arsenism and CYP1A1 and CYP2E1 gene polymorphism.MethodsCYP1A1 and CYP2E1 genotypes were determined with polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP) among 130 arsenism patients(cases) and 140 healthy people(controls).ResultsThe distribution frequencies of m1/m1and m1/m2+m2/m2 genotype ofCYP1A1 Msp I polymorphism site were 34.2% and 65.8% in cases and 30.9% and 69.1% in controls.The distribution frequencies of c1/c1 and c1/c2+c2/c2 genotype of CYP2E1 Pst I polymorphism site were 62.6% and 37.4% in cases and 57.4% and 42.6% in controls.The distribution frequencies of DD and DC+CC genotype of CYP2E1 Dra I polymorphism site were 58.7% and 41.3% in cases and 61.4% and 42.6% in controls.The distribution frequencies of all the genotypes were not significantly different between the cases and the controls(P=0.341,0.574,and 0.972).This study didn't find combined effects of CYP1A1 Msp I,CYP2E1 Pst I and CYP2E1 Dra I polymorphisms on the risk of coal burning-induced arsenism(P_adj= 0.433,0.219,and 0.832).ConclusionCYP1A1 Msp I,CYP2E1 Dra I and PstI polymorphism are not likely to associate with the risk of coal burning-induced arsenism.